The fact that the brain, this soft, unremarkable-looking tissue, is responsible for everything that makes us who we are, from our thinking to our behavior, seems mystical. However, just like the rest of our universe, the brain is also made of molecules subject to the laws of physics and physical chemistry, and it can be studied as such. This is the essential thinking behind 'biophysics'. Every physical aspect of the brain, from its wiring to molecule diffusion, has the potential to impact its function. Brain function is enabled by the culmination of intricate machinery that takes advantage of these biophysical properties. For instance, synaptic transmission, which lies at the core of brain function, is governed by the nano-metric synapse shape and the distribution of key molecules (i.e., synaptic architecture). During synaptic plasticity, which underlies learning and memory, the synaptic architecture is dynamically re-configured to enhance synaptic transmission. However, synaptic shape and molecular distribution are so small and elaborate that its study requires resolution beyond the diffraction limit. We use super-resolution microscopy techniques to overcome this barrier and explore the brain's microcosm.